Effect of fiber diameter and orientation on fibroblast morphology and proliferation on electrospun poly(d,l-lactic-co-glycolic acid) meshes

摘要: Engineered ligament tissues are promising materials for the repair of tears and ruptures, but require development biomaterial scaffolds that not only support physiologically relevant loads, also possess architectures capable orienting cell adhesion extracellular matrix deposition. Based on evidence micron-scale topographic features induce orientation through a contact guidance phenomenon, we postulate oriented fiber meshes-formed by electrospinning process-can regulate morphology. To test this, fused meshes poly(d,l-lactic-co-glycolic acid) (PLGA) were electrospun onto rigid supports under conditions produced mean diameters 0.14-3.6 microm, angular standard deviations 31-60 degrees . Analysis morphology adherent NIH 3T3 fibroblasts indicated projected area aspect ratio increased systematically with both increasing diameter degree orientation. Importantly, 3.6 microm fibers was similar to spincoated PLGA films. Finally, densities significantly different from PLGA, indicating proliferation is sensitive or