作者: Timothy Sibanda , Ramganesh Selvarajan , Memory Tekere , Hlengilizwe Nyoni , Stephen Meddows-Taylor
DOI: 10.1002/MBO3.498
关键词: Ulocladium 、 Trichoderma 、 Rhizopus 、 Microbiology 、 Mycobiota 、 Penicillium 、 Benzanthracene 、 Cellulase 、 Cladosporium 、 Biology
摘要: Urban life has created man-made extreme environments like carwashes. These have, however, not been sufficiently explored for mycobiota that can be sources of biotechnologically useful products, as the case with natural environments. Using a combination culture and molecular techniques, fungi from carwash effluents was characterized production lipase cellulase enzymes, nonpolar polar relevant secondary metabolites hydrocarbon utilization. The isolated fungal strains belonged to genera Alternaria, Cladosporium, Penicillium, Peyronellaea, Rhizopus, Spegazzinia, Trichoderma, Ulocladium Yarrowia. Sixty-six percent (66%) isolates were found able metabolize naphthalene benzanthracene, showing potential application in bioremediation polluted sites. Lipase by Penicillium sp. BPS3 (2.61 U/ml), Trichoderma BPS9 (2.01 U/ml), Rhizopus CAL1 (2.05 U/ml), PCW1 (2.99 U/ml) SAS1 (2.16 U/ml) compared well previously recorded levels other fungi. highest producers (12.10 U/ml), Peyronella CAW5 (4.49 U/ml) Cladosporium SAS3 (4.07 U/ml), although these activities lower than reported levels. GC-MS analysis resulted identification 572 compounds, including azulene, methanamine, N-pentylidene, metoclopramide, mepivacaine while compounds determined UHPLC-MS included 10-undecen-1-ol, piquerol A, 10-undecyn-1-ol, cyclo(leucylprolyl) rac-etomidate. have various anticancer, antibacterial, antifungal, antihypertensive, antidiabetic anti-inflammatory properties. study demonstrated are some important products.