Intravital Microscopy Reveals Novel Antivascular and Antitumor Effects of Endostatin Delivered Locally by Alginate-encapsulated Cells

摘要: The current study describes new, antivascular, and antitumor effects of human endostatin. A novel system for continuous, localized delivery antiangiogenic compounds to brain tumors was used. composed endostatin-producing 293 cells encapsulated into immuno-isolating sodium alginate. Intravital multifluorescence microscopy used assess vascular endostatin in C6 glioma spheroids implanted an ectopic as well orthotopic setting. Analysis total functional density, microvascular diameters, vessel perfusion, tumor growth, cell migration were performed repetitively. Tumor growth reduced by 35% treated animals. It interest that invasion the surrounding tissue also inhibited. density 67.6%, perfusion 67%, diameters 37%. This resulted a significant reduction although permeability not influenced. We have demonstrated only reduces shown previously, but greatly functionality vessels. Furthermore, we show this therapeutic approach inhibits invasion, thus supporting hypothesis angiogenesis represent two interrelated processes. Finally, work further confirms new concept using alginate cell-encapsulation technology central nervous malignancies.