Angiogenesis, Microvascular Architecture, Microhemodynamics, and Interstitial Fluid Pressure during Early Growth of Human Adenocarcinoma LS174T in SCID Mice

摘要: To date, most quantitative information on tumor angiogenesis, microcirculation, and transport has been derived from rodent tumors grown in transparent chamber preparations. In this paper we present a technique adapted to immunodeficient mice for the study of human xenografts. Microcirculatory parameters severe combined bearing dorsal skin fold preparation were quantified using intravital microscopy image analysis. The take rate colon adenocarcinoma LS174T was 100%, area doubling time 6.5 days. Three days following implantation 2 x 10(5) cells onto striated muscle, capillary sprouts noted cell mass. Microvasculature established after 10 Capillary density, vessel diameter, red blood velocity, flow rates individual microvessels measured 10, 14, 18, 22 showed no statistical difference muscle (capillaries) subcutaneous tissue (arterioles venules) tumor-free animals (N = 6), whereas these increased slightly, but not significantly, 7). Mean interstitial fluid pressure (+/- SD) small 4.6 +/- 1.7 mmHg 4) day 5.1 0.9 significantly elevated compared that (-0.9 0.8 mmHg) (P < 0.001). our knowledge, is first model enabling microscopic studies xenografts mice. Studies such should provide new insights into microcirculation-mediated mechanisms cancer treatment.