Models for assessment of angiogenesis in gliomas.

摘要: In the last two decades, much attention has been focussed on mechanisms of glioma vascularization including investigation growth factors and receptors involved. Recently, these efforts resulted in various approaches for antiangiogenic treatment experimental brain tumors. These basic science preclinical trials need an assortment models, which should allow investigating a variety questions. Several objectives concerning endothelial cell (EC) characteristics can adequately be studied vitro using EC monolayer assays. Three-dimensional spheroid techniques respect more complex cell–cell cell–environment interplay within three-dimensional culture. To optimize imitation crucial interaction human gliomas with host cells, immunological cells extracellular matrix, animal models are mandatory. An essential rule is to utilize orthotopic model, since tumor–host organ specific. avoid alloimmunogenic responses, it desirable use weakly or not immunogenic grafts, what best accomplished syngeneic model. However, rat poorly resemble patterns, xenografting into immunocompromized animals considered. vivo monitoring like videoscopy via cranial window magnetic resonance imaging (MRI) functional studies improve validity model employed. Finally, essentially recognize limitations each considered select that seems most appropriate investigated.