Potent synergism between vascular endothelial growth factor and basic fibroblast growth factor in the induction of angiogenesis in vitro

摘要: Vascular endothelial growth factor (VEGF), also known as vascular permeability or vasculotropin, is a recently characterized endothelial-specific mitogen which angiogenic in vivo. Here we demonstrate that VEGF vitro: when added to microvascular cells grown on the surface of three-dimensional collagen gels, induces invade underlying matrix and form capillary-like tubules, with an optimal effect at approximately 2.2nM (100ng/ml). When compared basic fibroblast (bFGF) equimolar (0.5nM) concentrations, was about half potent. The most striking seen combination bFGF: simultaneously, bFGF induced vitro response far greater than additive, occurred rapidity either cytokine alone. These results like bFGF, via direct cells, by acting concert, these two cytokines have potent synergistic induction angiogenesis vitro. We suggest synergism between plays important role control