Models for angiogenesis in gliomas.

摘要: During the last decades a lot of attention has been focussed on mechanisms glioma vascularization, particularly in terms investigating vascular growth factors and receptors. Recently, these efforts resulted various approaches for antiangiogenic treatment strategies using vitro cell culture systems as well experimental orthotopic non-orthotopic brain tumors. These basic science preclinical trials need an assortment models, which should allow variety questions. Several objectives concerning endothelial (EC) characteristics can adequately be studied EC monolayer assays. Three - dimensional spheroid techniques respect more complex cell-cell cell-environment interplay within 3-dimensional culture. Recent advances molecular genetic offer wide access to genome EC. Using micro array or chip methods differences between micro-and macromolecular variations gene pool different organ specific assessed. To optimize imitation crucial interaction human gliomas with host cells, immunological cells extracellular matrix, animal models are mandatory. An essential rule is utilize model, since tumor-host-interaction specific. avoid alloimmunogenic responses, it desirable use weak non-immunogenic grafts, best accomplished syngeneic model. However, rat poorly resemble patterns, xenografting into immunocompromized animals considered. In vivo-monitoring like videoscopy via cranial window magnetic resonance imaging (MRI) functional studies improve validity model employed. Finally, essentially recognize limitations each considered select that seems most appropriate investigated.