A novel vascular endothelial growth factor encoded by Orf virus, VEGF‐E, mediates angiogenesis via signalling through VEGFR‐2 (KDR) but not VEGFR‐1 (Flt‐1) receptor tyrosine kinases

摘要: The different members of the vascular endothelial growth factor (VEGF) family act as key regulators cell function controlling vasculogenesis, angiogenesis, permeability and survival. In this study, we have functionally characterized a novel member VEGF family, designated VEGF-E. VEGF-E sequences are encoded by parapoxvirus Orf virus (OV). They carry characteristic cysteine knot motif present in all mammalian VEGFs, while forming microheterogenic group distinct from previously described family. was expressed native protein cells or recombinant Escherichia coli shown to heat-stable, secreted dimer. VEGF-A were found possess similar bioactivities, i.e. both factors stimulate release tissue (TF), proliferation, chemotaxis sprouting cultured vitro angiogenesis vivo. Like VEGF-A, bind with high affinity receptor-2 (KDR) resulting receptor autophosphorylation biphasic rise free intracellular Ca2+ concentration, whilst contrast did not receptor-1 (Flt-1). is thus potent angiogenic selectively binding receptor-2. These data strongly indicate that activation alone can efficiently angiogenesis.