Glioma cell migration is associated with glioma-induced angiogenesis in vivo.

摘要: To simultaneously assess glioma cell invasion and angiogenesis in vivo by non-invasive quantitative means, DiI-labeled C6 spheroids were implanted into the dorsal skinfold chamber preparation of nude mice (n = 6). Heat-inactivated served as controls to distinguish between active passive spread. Using multi-fluorescent intravital videomicroscopy, migration was analyzed on days 1-4, 6, 10 spheroid vascularization 3, after implantation. Additionally, chronic cranial window an orthotopic implantation site 4). In chamber, vascularized within revealed a tumor-specific microvasculature. parallel, individual cells detached from edge migrated centrifugally demonstrating affinity tumor host vessels. Glioma demonstrated heterogeneous pattern their regional migratory activity (0.2-9.6 microm/h) which correlated well with (r 0.733). window, spread similarly perivascular space pial/subpial vessels preference arteriolar segments. Intravital fluorescence microscopy represents versatile technique complex relationship glioma-driven invasion.