Assessment of brain tumor cell motility in vivo and in vitro

摘要: Brain tumor dispersal far from bulk contributes to and, in some instances, dominates disease progression. Three methods were used characterize brain cell motility vivo and vitro: 1) 2 weeks after implantation rat cerebral cortex, single C6 cells labeled with a fluorescent tag had migrated sites greater than 16 mm distant tumor; 2) time-lapse videomicroscopy of human revealed 12.5 microns/hr. Ruffling leading edges pseudopod formation most elaborate more malignant cells; 3) an vitro assay was devised quantitatively evaluate region high density one lower density. Human plated the center petri dish, washed, refed, establishing 2-cm circular zone dish center. Motility determined by counting daily at predetermined distances central perimeter. Cells found 1 cm perimeter 24 hours 3 4 days. Increasing serum concentration increased motility; however, neither fibronectin nor arrest G0 phase hydroxyurea altered motility. The addition cytochalasin B block cytoskeletal assembly prevented malignancy. Subpopulations created clonal amplification that rapidly periphery. Although morphologically indistinguishable when compared original line which they derived, these subpopulations demonstrated significantly