Long-term serial cultivation of arterial and capillary endothelium from adult bovine brain.

摘要: Cerebrovascular endothelial cells from adult bovine brain were carried successfully in long-term, serial culture. Endothelial obtained the middle and anterior cerebral arteries capillaries isolated grey matter of cortex or caudate nucleus. Capillary found to grow best RPMI 1640 with 20% fetal serum. They did not require tumor-conditioned medium matrix-coated surfaces, although fibronectin was used enhance initial plating efficiency primary cultures. The same conditions support satisfactory growth arterial cells; however they as rapidly capillary cells. Retention endothelial-specific characteristics shown for capillary-derived up Passage 28, arterial-derived 11, after frozen storage both types cultured Cultures stained positively Factor VIII antigen, exhibited a nonthrombogenic surface, produced prostacyclin response arachidonic acid. Arterial more than endothelium. had unique tendency assume ringlike morphology subculture sometimes formed capillarylike networks cell cords dense When three-dimensional plasma clot, cells, but none other studied, organized into tubelike structures reminiscent formation vivo. availability long-term cultures cerebrovascular provides an opportunity compare properties endothelium tissue investigate such processes angiogenesis blood-brain barrier induction.