Astrocytes induce neural microvascular endothelial cells to form capillary-like structures in vitro.

摘要: Astrocytes maintain a unique association with the central nervous system microvasculature and are thought to play role in neural microvessel formation differentiation. We investigated influence of astroglial cells on microvascular endothelial differentiation vitro. Using an astroglial-endothelial coculture system, rat brain astrocytes C6 lineage shown induce bovine retinal (BRE) form capillary-like structures. Light microscopic evidence for reorganization began within 48 hours was complete 72-96 following addition BRE 1-day-old cultures. The extent quantitated by computer-assisted analysis be dependent upon density both cocultures. Coculture conditions which were separated from porous membranes failed generate this cell change. Likewise, C6-conditioned media C6-endothelial conditioned did not reorganization. Extracellular laminin cocultures, identified indirect immunofluorescence, concentrated at endothelial-astroglial interface structures consistent incipient basement membrane formation. Astroglial accumulated adjacent suggesting presence bidirectional influences between reorganized astroglia. This is first demonstration induction angiogenesis vitro these findings support functional perivascular vascularization tissue such as retina brain.