A quantitative promoter methylation profile of prostate cancer.

摘要: Purpose: Promoter hypermethylation is an alternative pathway for gene silencing in neoplastic cells and a promising cancer detection marker. Although quantitative methylation-specific PCR (QMSP) of the GSTP1 promoter has demonstrated near perfect specificity prostate biopsies, we postulated that identification characterization additional methylation markers might further improve its high (80–90%) sensitivity. Experimental Design: We surveyed nine promoters ( , MGMT p14 / ARF p16 CDKN2A RASSF1A APC TIMP3 S100A2 CRBP1 ) by QMSP tissue DNA from 118 carcinomas, 38 paired high-grade prostatic intraepithelial neoplasias (HGPIN), 30 benign hyperplasias (BPH). The levels were calculated correlated with clinical pathologic indicators. Results: Only frequencies significantly higher carcinoma compared BPH P differed between HGPIN, and/or HGPIN or theoretical sensitivity 98.3% carcinoma, 100% specificity. Methylation found to correlate tumor grade stage ). Conclusions: Our data demonstrate existence progressive increase several cancer-related genes carcinogenesis, providing augment molecular carcinoma. Because are associated advanced stage, indicators currently used predict aggressiveness.