An intranuclear bacilliform virus associated with near extirpation of Austropotamobius pallipes Lereboullet from the Nant watershed in Ardeche, France

摘要: White-clawed crayfish, Austropotamobius pallipes, were endemic to the Nant watershed, Ardeche, France, until they extirpated by epizootic mortality at beginning of twentieth century. A. pallipes successfully reintroduced watershed in middle However, was observed summer 2000 during which time from downstream regions. Dead and moribund crayfish again detected several episodes 2001 October range reduced headwaters just one three streams watershed. Water quality for appropriate habitation. Bacteriology mycology on collected failed reveal a cause. histopathology revealed high occurrence intranuclear eosinophilic inclusions hepatopancreatocytes pallipes. The nuclei hypertrophic contained bacilliform virions consisting cylindrical nucleocapsid surrounded trilaminar envelope. Virions section approximately 63 x 258 nm nucleocapsids 52 225 nm. It is unclear whether virus cause or contributor disease complex involving other undetected pathogens.