Antidiarrheal and antispasmodic activities of Vincetoxicum stocksii are mediated through calcium channel blockade
作者： Abdul Jabbar Shah , Mudassar A. Zaidi , Hassan Sajjad , . Hamidullah , Anwarul-Hassan Gilani
关键词: Jejunum 、 Antispasmodic 、 Contraction (grammar) 、 Vincetoxicum stocksii 、 Calcium channel blockade 、 Verapamil 、 Calcium channel 、 Internal medicine 、 Chemistry 、 Endocrinology 、 Loperamide 、 Pharmacology
摘要: This study was carried out to explore the mechanism underlying antidiarrheal and antispasmodic activities of Vincetoxicum stocksii . The crude extract V. provided 12-24% protection from castor oil-induced diarrhea at dose 300-1,000 mg/kg, similar loperamide. In isolated rabbit jejunum preparations, caused inhibition spontaneous high K + (80 mM)-induced contractions, with respective EC 50 values 2.53 (1.65-3.90) 0.95 mg/mL (0.63-1.42), that by verapamil, suggesting calcium channel blocking effect. Loperamide -induced contraction, 8.59 (6.33-10.11) 9.12 µM (7.33-12.81). blocking activity further confirmed when pretreatment tissues (1-3 mg/mL) a rightward displacement Ca ++ concentrations response curves, produced verapamil; constructed in free medium. These data indicate contains constituents may possibly explain its medicinal use hyperactive states gut, such as, spasms.
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sci-hub.se 参考文章(17)
Tiziano Croci, Marco Landi, Xavier Emonds-Alt, Gérard Le Fur, Jean-Pierre Maffrand, Luciano Manara, Role of tachykinins in castor oil diarrhoea in rats British Journal of Pharmacology. ,vol. 121, pp. 375- 380 ,(1997) , 10.1038/SJ.BJP.0701130
A. H. Gilani, F. Shaheen, S. A. Saeed, Cardiovascular actions ofDaucus carota Archives of Pharmacal Research. ,vol. 17, pp. 150- 153 ,(1994) , 10.1007/BF02974250
M. E. Wall, C. R. Eddy, M. L. McClennan, M. E. Klumpp, Detection and Estimation of Steroidal Sapogenins in Plant Tissue Analytical Chemistry. ,vol. 24, pp. 1337- 1341 ,(1952) , 10.1021/AC60068A018
Judy Ann Bigby, Goodman and Gillman's The Pharmacological Basis of Therapeutics Archives of Dermatology. ,vol. 128, pp. 132- 132 ,(1992) , 10.1001/ARCHDERM.1992.01680110146030
Mudassir A. Zaidi, Sidney A. Crow, Biologically active traditional medicinal herbs from Balochistan, Pakistan. Journal of Ethnopharmacology. ,vol. 96, pp. 331- 334 ,(2005) , 10.1016/J.JEP.2004.07.023
F. AWOUTERS, C. J. E. NIEMEGEERS, F. M. LENAERTS, P. A. J. JANSSEN, DELAY OF CASTOR OIL DIARRHOEA IN RATS: A NEW WAY TO EVALUATE INHIBITORS OF PROSTAGLANDIN BIOSYNTHESIS Journal of Pharmacy and Pharmacology. ,vol. 30, pp. 41- 45 ,(2011) , 10.1111/J.2042-7158.1978.TB13150.X
Anwarul Hassan Gilani, Abdul Jabbar Shah, Muhammad Nabeel Ghayur, Kashif Majeed, Pharmacological basis for the use of turmeric in gastrointestinal and respiratory disorders. Life Sciences. ,vol. 76, pp. 3089- 3105 ,(2005) , 10.1016/J.LFS.2004.12.021
Min Zhu, J. David Phillipson, Pam M. Greengrass, Norman E. Bowery, Ya Cai, Plant polyphenols: biologically active compounds or non-selective binders to protein? Phytochemistry. ,vol. 44, pp. 441- 447 ,(1997) , 10.1016/S0031-9422(96)00598-5
A.J. Farre, M. Colombo, M. Fort, B. Gutierrez, Differential effects of various Ca2+ antagonists General Pharmacology: The Vascular System. ,vol. 22, pp. 177- 181 ,(1991) , 10.1016/0306-3623(91)90331-Y
Abdul Jabbar Shah, Nizar Noorali Bhulani, Sara Haroon Khan, Najeeb ur Rehman, Anwarul Hassan Gilani, Calcium channel blocking activity of Mentha longifolia L. explains its medicinal use in diarrhoea and gut spasm Phytotherapy Research. ,vol. 24, pp. 1392- 1397 ,(2010) , 10.1002/PTR.3263