Lhx2 Expression Promotes Self-Renewal of a Distinct Multipotential Hematopoietic Progenitor Cell in Embryonic Stem Cell-Derived Embryoid Bodies

摘要: The molecular mechanisms regulating the expansion of hematopoietic system including stem cells (HSCs) in fetal liver during embryonic development are largely unknown. LIM-homeobox gene Lhx2 is a candidate regulator hematopoiesis since it expressed and Lhx2−/− mice die utero due to severe anemia. Moreover, expression (ES) cell-derived embryoid bodies (EBs) can lead generation HSC-like cell lines. To further define role this transcription factor regulation, we generated ES lines that enabled tet-inducible Lhx2. Using approach observed synergises with specific signalling pathways, resulting increased frequency colony forming developing EB cells. increase growth factor-responsive progenitor directly correlates efficiency generating lines, suggesting induce self-renewal distinct multipotential EBs. Signalling via c-kit tyrosine kinase receptor gp130 signal transducer by IL-6 necessary sufficient for induced self-renewal. While inducing cells, inhibited proliferation primitive erythroid precursor interfered early commitment, indicating striking lineage specificity effect.