Cytotoxic Impact of Costunolide Isolated from Costus speciosus on Breast Cancer via Differential Regulation of Cell Cycle-An In-vitro and In-silico Approach.
作者： Anita Roy , Rajalakshmi Manikkam
DOI: 10.1002/PTR.5408
关键词: Cyclin D1 、 Pharmacology 、 Cell division 、 Costunolide 、 Apoptosis 、 Cell cycle checkpoint 、 Cancer cell 、 Cell cycle 、 Chemistry 、 Cell growth
摘要: Costunolide, a sesquiterpene lactone, is biologically active molecule found in most of the medicinally valuable plants. The present study aims to evaluate anticancer property costunolide isolated from Costus speciosus against breast cancer cell lines (MCF-7 and MDA-MB-231). Costunolide effectively reduced viability both MCF-7 MDA-MB-231 at an IC50 value 40 μM. Flow cytometric analysis revealed mediated cycle arrest G2/M phase types. Western blotting results confirmed alterations expression regulators (cyclin D1, D3, CDK-4, CDK-6, p18 INK4c, p21 CIP1/Waf-1 p27 KIP1) apoptosis inducers (caspase-3 caspase-9) upon treatment comparison with their expressions normal line (MCF-10A). downregulation positive upregulation negative were related induction cells. above validated in-silico that predicted stable interactions between targets. Thus induced targeting regulation, compound can be used as effective herbal therapeutic treat further explorations.
sci-hub.se 参考文章(31)
David Lydall, Ted Weinert, Cell cycle checkpoints, genetic instability and cancer. Seminars in Cancer Biology. ,vol. 4, pp. 129- 140 ,(1993)
Chenguan Wang, Zhiping Li, Maofu Fu, Toula Bouras, Richard G. Pestell, Signal transduction mediated by cyclin D1: from mitogens to cell proliferation: a molecular target with therapeutic potential. Cancer treatment and research. ,vol. 119, pp. 217- 237 ,(2004) , 10.1007/1-4020-7847-1_11
Graña X, Reddy Ep, Cell cycle control in mammalian cells : role of cyclins, cyclin dependent kinases (CDKs), growth suppressor genes and cyclin-dependent kinase inhibitors (CKIs) Oncogene. ,vol. 11, pp. 211- 219 ,(1995)
Reiner U. Jänicke, Quinn L. Deveraux, Stanislaw Krajewski, Alan G. Porter, Rolf Jaggi, Andreas Marti, Céline Blanc, John C. Reed, Caspase-3 is essential for procaspase-9 processing and cisplatin-induced apoptosis of MCF-7 breast cancer cells. Cancer Research. ,vol. 60, pp. 4386- 4390 ,(2000)
A. Lewis Farr, Oliver H. Lowry, Rose J. Randall, Nira J. Rosebrough, Protein Measurement with the Folin Phenol Reagent Journal of Biological Chemistry. ,vol. 193, pp. 265- 275 ,(1951)
A. Carnero, G. J. Hannon, The INK4 family of CDK inhibitors Current Topics in Microbiology and Immunology. ,vol. 227, pp. 43- 55 ,(1998) , 10.1007/978-3-642-71941-7_3
Hikaru Izawa, Hirofumi Yamamoto, Bazarragchaa Damdinsuren, Kimimasa Ikeda, Masaki Tsujie, Rei Suzuki, Kotaro Kitani, Yosuke Seki, Taro Hayashi, Ichiro Takemasa, Masataka Ikeda, Masayuki Ohue, Mitsugu Sekimoto, Takushi Monden, Morito Monden, None, Effects of p21cip1/waf1 overexpression on growth, apoptosis and differentiation in human colon carcinoma cells. International Journal of Oncology. ,vol. 27, pp. 69- 76 ,(2005) , 10.3892/IJO.27.1.69
Adrian Russell, M Anne Thompson, Joy Hendley, Lyn Trute, Jane Armes, Doris Germain, Cyclin D1 and D3 associate with the SCF complex and are coordinately elevated in breast cancer Oncogene. ,vol. 18, pp. 1983- 1991 ,(1999) , 10.1038/SJ.ONC.1202511
Colin Adrain, Seamus J Martin, The mitochondrial apoptosome: a killer unleashed by the cytochrome seas. Trends in Biochemical Sciences. ,vol. 26, pp. 390- 397 ,(2001) , 10.1016/S0968-0004(01)01844-8
M H Lee, I Reynisdottir, J Massague, Cloning of p57KIP2, a cyclin-dependent kinase inhibitor with unique domain structure and tissue distribution. Genes & Development. ,vol. 9, pp. 639- 649 ,(1995) , 10.1101/GAD.9.6.639