Effects of p21cip1/waf1 overexpression on growth, apoptosis and differentiation in human colon carcinoma cells.

摘要: The cyclin-dependent kinase inhibitor p21 cip1/waf1 negatively regulates the progression of cell cycle and potential usefulness gene is proposed in therapy. However, studies have demonstrated a protective role against apoptosis little known about effects ectopic expression on differentiation colon cancer cells. In present study, we found diffuse only few clinical samples colorectal with wild-type p53 gene. To explore growth, differentiation, constitutively overexpressed HT29 carcinoma Ectopic overexpression was associated inhibition CDK2-associated activity, indicating functionality introduced Overexpression caused an appreciable growth monolayer soft agar cultures it significantly reduced sodium butyrate- but not 5-fluorouracil-induced apoptosis. overexpressing cells exhibited marked decrease intestinal when assayed alkaline phosphatase. Our findings suggest that introduction into may be useful for inhibiting caution should taken regarding increased resistance to certain apoptosis-inducing agents dysregulation endogenous -mediated process.