Induction of cancer cell-specific death via MMP2 promoterdependent Bax expression.
作者： Eun-Jeong Seo , Se-Woon Kim , Eek-hoon Jho
DOI: 10.5483/BMBREP.2009.42.4.217
关键词:
摘要: Controlled gene expression in specific cells is a valuable tool for therapy. We attempted to determine whether the lentivirus-mediated Tet-On inducible system could be applied cancer In order select genes that induce cell death, we compared ability of known pro-apoptotreic genes, Bax and tBid, cycle inhibitor, p21cip1/waf1, determined was most effective. For cell-specific rtTA2(S)-M2, tested matrix metalloproteinase-2 (MMP-2) promoter it highly expressed lines, including SNU475 cells. The co-transduction two lentiviruses contain sequences TRE-Bax which controlled by MMP-2 promoter, resulted death SNU475, whereas other with low did not evidence significant death. Our data indicate using cancer-specific applicable
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