Transcriptional targeting of gene expression in breast cancer by the promoters of protein regulator of cytokinesis 1 and ribonuclease reductase 2

摘要: For cancer gene therapy, cancer-specific over-expression of a therapeutic is required to reduce side effects derived from expression the in normal cells. To develop such an vector, we searched for genes over-expressed and/or specifically expressed cells using bioinformatics and have selected coding protein regulator cytokinesis 1 (PRC1) ribonuclease reductase 2 (RRM2) as candidates. Their expressions were confirmed both breast cell lines patient tissues. We compared each promoter's activity luciferase reporter PRC1 RRM2 promoters. test activities these promoters viral vectors, also cloned into adeno-associated (AAV) vector containing green fluorescence (GFP) reporter. The GFP levels by various depending on tested and, MDA-MB-231 cells, strong that cytomegalovirus (CMV) promoter. Our result showed or promoter could be used specific overexpression therapy.