Absence of the vagus nerve in the stomach of Tbx1-/- mutant mice.

作者: A. Calmont , N. Thapar , P. J. Scambler , A. J. Burns

DOI: 10.1111/J.1365-2982.2010.01615.X

关键词: PathologyEnteric nervous systemGastrointestinal functionStomachGanglion formationCardiac neural crest cellsTBX1MedicineNeural crestAnatomyVagus nerve

摘要: Background  Tbx1 is a member of the Tbox family binding domain transcription factors. TBX1 maps within region chromosome 22q11 deleted in humans with DiGeorge syndrome (DGS), common genetic disorder characterized by numerous physical manifestations including craniofacial and cardiac anomalies. Mice homozygous null mutations phenocopy this have defects abnormal cranial ganglia formation neural crest cell migration. These prompted us to investigate whether extrinsic vagus nerve or intrinsic enteric nervous system abnormalities are prevalent gastrointestinal tract mutant mice. Methods  We used situ hybridization for Ret, immunohistochemical staining neurofilament, HuC/D βIII-tubulin study ganglia, nerve, development control mice. Key Results  In Tbx1−/− embryos, glossopharyngeal (IXth) (Xth) nerves were malformed abnormally fused. tract, adjacent esophagus severely hypoplastic they did not extend beyond gastro-esophageal junction nor project branches stomach wall, as was observed Tbx1+/+ mice. Conclusions & Inferences  Although morphology appeared normal Tbx1+/− mice, these animals had spectrum innervation ranging from mild severe. all genotypes, developed normally. The deficit vagal mice gene implicated DGS raises possibility that similar may underlie number yet unidentified/unreported congenital disorders affecting function.

参考文章(11)
Elizabeth A. Lindsay, Francesca Vitelli, Hong Su, Masae Morishima, Tuong Huynh, Tiziano Pramparo, Vesna Jurecic, George Ogunrinu, Helen F. Sutherland, Peter J. Scambler, Allan Bradley, Antonio Baldini, Tbx1 haploinsufficiency in the DiGeorge syndrome region causes aortic arch defects in mice Nature. ,vol. 410, pp. 97- 101 ,(2001) , 10.1038/35065105
Masumi Takano-Maruyama, Yiju Chen, Gary O. Gaufo, Placodal sensory ganglia coordinate the formation of the cranial visceral motor pathway Developmental Dynamics. ,vol. 239, pp. 1155- 1161 ,(2010) , 10.1002/DVDY.22273
Peggy S. Eicher, Donna M. McDonald-McGinn, Catherine A. Fox, Deborah A. Driscoll, Beverly S. Emanuel, Elaine H. Zackai, Dysphagia in children with a 22q11.2 deletion: unusual pattern found on modified barium swallow. The Journal of Pediatrics. ,vol. 137, pp. 158- 164 ,(2000) , 10.1067/MPD.2000.105356
Francesca Vitelli, Masae Morishima, Ilaria Taddei, Elizabeth A Lindsay, Antonio Baldini, Tbx1 mutation causes multiple cardiovascular defects and disrupts neural crest and cranial nerve migratory pathways Human Molecular Genetics. ,vol. 11, pp. 915- 922 ,(2002) , 10.1093/HMG/11.8.915
Peter J. Scambler, 22q11 Deletion Syndrome: A Role for TBX1 in Pharyngeal and Cardiovascular Development Pediatric Cardiology. ,vol. 31, pp. 378- 390 ,(2010) , 10.1007/S00246-009-9613-0
Eva Coppola, Murielle Rallu, Juliette Richard, Sylvie Dufour, Dieter Riethmacher, François Guillemot, Christo Goridis, Jean-François Brunet, Epibranchial ganglia orchestrate the development of the cranial neurogenic crest Proceedings of the National Academy of Sciences of the United States of America. ,vol. 107, pp. 2066- 2071 ,(2010) , 10.1073/PNAS.0910213107
Alan J. Burns, Migration of neural crest-derived enteric nervous system precursor cells to and within the gastrointestinal tract The International Journal of Developmental Biology. ,vol. 49, pp. 143- 150 ,(2005) , 10.1387/IJDB.041935AB
Clare V.H. Baker, Marianne Bronner-Fraser, Vertebrate cranial placodes I. Embryonic induction. Developmental Biology. ,vol. 232, pp. 1- 61 ,(2001) , 10.1006/DBIO.2001.0156
Elyanne M. Ratcliffe, Suhas U. Setru, Jason J. Chen, Zhishan S. Li, Fabien D'Autréaux, Michael D. Gershon, Netrin/DCC-mediated attraction of vagal sensory axons to the fetal mouse gut. The Journal of Comparative Neurology. ,vol. 498, pp. 567- 580 ,(2006) , 10.1002/CNE.21027
A. Calmont, S. Ivins, K. L. Van Bueren, I. Papangeli, V. Kyriakopoulou, W. D. Andrews, J. F. Martin, A. M. Moon, E. A. Illingworth, M. A. Basson, P. J. Scambler, Tbx1 controls cardiac neural crest cell migration during arch artery development by regulating Gbx2 expression in the pharyngeal ectoderm. Development. ,vol. 136, pp. 3173- 3183 ,(2009) , 10.1242/DEV.028902