MicroRNA-221 silencing predisposed human bladder cancer cells to undergo apoptosis induced by TRAIL

摘要: Abstract Objectives Bladder cancer is the most common type of urologic in Chinese males. The 5-year survival rate advanced bladder approximately 20%–40%. There an obvious urgent need for novel and effective therapies against cancer. MicroRNAs (miRNAs) are a recently discovered class noncoding RNAs; suppressing miRNA-221 might prove beneficial several cancers. To explore cancer, we explored effects silencing on cells. Materials methods Northern blot analysis was used to determine expression levels T24 cells, RT4 cells human normal urothelial silenced with antisense oligonucleotides pro-apoptotic effect necrosis factor related apoptosis-inducing ligand (TRAIL) miRNA-221-silenced assessed flow cytometry. p27kip1 protein exposed TRAIL detected by Western blotting. role cell cycle phase distribution investigated through cytometric analysis. Results Human significantly up-regulated compared TRAIL-resistant line. MiRNA-221 predisposed undergo apoptosis induced resulted up-modulation cyclin-dependent kinase inhibitor p27Kip1. suppression promoted activation caspase 3 Conclusions rendered TRAIL. Our findings suggest potential therapy.