A FancD2-Monoubiquitin Fusion Reveals Hidden Functions of Fanconi Anemia Core Complex in DNA Repair
作者： Nobuko Matsushita , Hiroyuki Kitao , Masamichi Ishiai , Naoki Nagashima , Seiki Hirano
DOI: 10.1016/J.MOLCEL.2005.08.018
关键词: FANCG 、 Biology 、 FANCD2 、 FAN1 、 DNA repair 、 Molecular biology 、 Cell biology 、 Chromatin 、 Fusion protein 、 Fanconi anemia, complementation group C 、 Monoubiquitination
摘要: In DNA damage responses, the Fanconi anemia (FA) protein, FancD2, is targeted to chromatin and forms nuclear foci following its monoubiquitination, a process likely catalyzed by FA core complex. Here, we show that chicken FancD2-ubiquitin fusion carrying Lys-Arg substitution removing natural monoubiquitination site (D2KR-Ub), could reverse cisplatin hypersensitivity localize in FANCD2-deficient DT40 cells. Importantly, targeting was dependent on three complex components as well hydrophobic surface of ubiquitin may direct protein-protein interactions. Furthermore, constitutively bound D2KR-histone H2B complement sensitivity FANCD2- but not FANCC-, FANCG-, or FANCL-deficient Thus these have an additional function repair, which independent FancD2. These results define functional consequences FancD2 reveal previously hidden functions for protein
elsevier.com
unirioja.es
core.ac.uk
sci-hub.se 参考文章(27)
Pier Paolo Di Fiore, Simona Polo, Kay Hofmann, When ubiquitin meets ubiquitin receptors: a signalling connection Nature Reviews Molecular Cell Biology. ,vol. 4, pp. 491- 497 ,(2003) , 10.1038/NRM1124
Guillaume Rigaut, Anna Shevchenko, Berthold Rutz, Matthias Wilm, Matthias Mann, Bertrand Séraphin, None, A generic protein purification method for protein complex characterization and proteome exploration. Nature Biotechnology. ,vol. 17, pp. 1030- 1032 ,(1999) , 10.1038/13732
Irene Garcia-Higuera, Toshiyasu Taniguchi, Shridar Ganesan, M.Stephen Meyn, Cynthia Timmers, James Hejna, Markus Grompe, Alan D D'Andrea, Interaction of the Fanconi anemia proteins and BRCA1 in a common pathway. Molecular Cell. ,vol. 7, pp. 249- 262 ,(2001) , 10.1016/S1097-2765(01)00173-3
Claudia Lukas, Fredrik Melander, Manuel Stucki, Jacob Falck, Simon Bekker-Jensen, Michal Goldberg, Yaniv Lerenthal, Stephen P Jackson, Jiri Bartek, Jiri Lukas, Mdc1 couples DNA double-strand break recognition by Nbs1 with its H2AX-dependent chromatin retention The EMBO Journal. ,vol. 23, pp. 2674- 2683 ,(2004) , 10.1038/SJ.EMBOJ.7600269
Sebastian M.B. Nijman, Tony T. Huang, Annette M.G. Dirac, Thijn R. Brummelkamp, Ron M. Kerkhoven, Alan D. D'Andrea, René Bernards, The Deubiquitinating Enzyme USP1 Regulates the Fanconi Anemia Pathway Molecular Cell. ,vol. 17, pp. 331- 339 ,(2005) , 10.1016/J.MOLCEL.2005.01.008
Claudia Lukas, Jacob Falck, Jirina Bartkova, Jiri Bartek, Jiri Lukas, Distinct spatiotemporal dynamics of mammalian checkpoint regulators induced by DNA damage Nature Cell Biology. ,vol. 5, pp. 255- 260 ,(2003) , 10.1038/NCB945
Woo-Hyun Park, Steven Margossian, Andrew A. Horwitz, Amanda M. Simons, Alan D. D'Andrea, Jeffrey D. Parvin, Direct DNA Binding Activity of the Fanconi Anemia D2 Protein Journal of Biological Chemistry. ,vol. 280, pp. 23593- 23598 ,(2005) , 10.1074/JBC.M503730200
Stephen C. West, Molecular views of recombination proteins and their control Nature Reviews Molecular Cell Biology. ,vol. 4, pp. 435- 445 ,(2003) , 10.1038/NRM1127
Ashok R. Venkitaraman, Tracing the network connecting BRCA and Fanconi anaemia proteins. Nature Reviews Cancer. ,vol. 4, pp. 266- 276 ,(2004) , 10.1038/NRC1321
Hiroyuki Nishikawa, Seido Ooka, Ko Sato, Kei Arima, Joji Okamoto, Rachel E. Klevit, Mamoru Fukuda, Tomohiko Ohta, Mass Spectrometric and Mutational Analyses Reveal Lys-6-linked Polyubiquitin Chains Catalyzed by BRCA1-BARD1 Ubiquitin Ligase Journal of Biological Chemistry. ,vol. 279, pp. 3916- 3924 ,(2004) , 10.1074/JBC.M308540200