Optimization of Droplet Digital PCR from RNA and DNA extracts with direct comparison to RT-qPCR: Clinical implications for quantification of Oseltamivir-resistant subpopulations

作者: Sean C. Taylor , Julie Carbonneau , Dawne N. Shelton , Guy Boivin

DOI: 10.1016/J.JVIROMET.2015.08.014

关键词: PopulationMolecular biologyNucleic acidBiologyNeuraminidaseStandard curveRNADNADigital polymerase chain reactionPolymerase chain reaction

摘要: … In a typical optimization experiment, a pooled RNA or cDNA sample (diluted at least 1/10) from all the study sample sets should initially be tested in RT-qPCR with all primers pairs and …

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