A Commercial Real-Time PCR Kit Provides Greater Sensitivity than Direct Sequencing to Detect KRAS Mutations
作者： Bárbara Angulo , Elena García-García , Rebeca Martínez , Ana Suárez-Gauthier , Esther Conde
DOI: 10.2353/JMOLDX.2010.090139
关键词:
摘要: … gene was performed using PCR and automatic direct sequencing as previously described.14, 15 Exon 2 of the KRAS gene was amplified in duplicate and all variants were confirmed by …
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