The Future of Digital Polymerase Chain Reaction in Virology.

作者: Matthijs Vynck , Wim Trypsteen , Olivier Thas , Linos Vandekerckhove , Ward De Spiegelaere

DOI: 10.1007/S40291-016-0224-1

关键词: Digital polymerase chain reactionVirologyAbsolute quantificationCurrent technologyClinical virologyBiology

摘要: Driven by its potential benefits over currently available methods, and the recent development of commercial platforms, digital polymerase chain reaction (dPCR) has received increasing …

参考文章(76)
John M. S. Bartlett, David Stirling, A short history of the polymerase chain reaction. Methods of Molecular Biology. ,vol. 226, pp. 3- 6 ,(2003) , 10.1385/1-59259-384-4:3
P Simmonds, P Balfe, J F Peutherer, C A Ludlam, J O Bishop, A J Brown, Human immunodeficiency virus-infected individuals contain provirus in small numbers of peripheral mononuclear cells and at low copy numbers. Journal of Virology. ,vol. 64, pp. 864- 872 ,(1990) , 10.1128/JVI.64.2.864-872.1990
Jernej Pavšič, Alison S. Devonshire, Helen Parkes, Heinz Schimmel, Carole A. Foy, Maria Karczmarczyk, Ion Gutiérrez-Aguirre, Isobella Honeyborne, Jim F. Huggett, Timothy D. McHugh, Mojca Milavec, Heinz Zeichhardt, Jana Žel, Standardization of Nucleic Acid Tests for Clinical Measurements of Bacteria and Viruses Journal of Clinical Microbiology. ,vol. 53, pp. 2008- 2014 ,(2015) , 10.1128/JCM.02136-14
Jernej Pavšič, Jana Žel, Mojca Milavec, Digital PCR for direct quantification of viruses without DNA extraction Analytical and Bioanalytical Chemistry. ,vol. 408, pp. 67- 75 ,(2016) , 10.1007/S00216-015-9109-0
Ming Yu, Kelly T Carter, Karen W Makar, Kathy Vickers, Cornelia M Ulrich, Robert E Schoen, Dean Brenner, Sanford D Markowitz, William M Grady, MethyLight droplet digital PCR for detection and absolute quantification of infrequently methylated alleles Epigenetics. ,vol. 10, pp. 803- 809 ,(2015) , 10.1080/15592294.2015.1068490
Motokazu Mukaide, Masaya Sugiyama, Masaaki Korenaga, Kazumoto Murata, Tatsuya Kanto, Naohiko Masaki, Masashi Mizokami, High-throughput and sensitive next-generation droplet digital PCR assay for the quantitation of the hepatitis C virus mutation at core amino acid 70 Journal of Virological Methods. ,vol. 207, pp. 169- 177 ,(2014) , 10.1016/J.JVIROMET.2014.07.006
Alison S. Devonshire, Isobella Honeyborne, Alice Gutteridge, Alexandra S. Whale, Gavin Nixon, Philip Wilson, Gerwyn Jones, Timothy D. McHugh, Carole A. Foy, Jim F. Huggett, Highly reproducible absolute quantification of Mycobacterium tuberculosis complex by digital PCR. Analytical Chemistry. ,vol. 87, pp. 3706- 3713 ,(2015) , 10.1021/AC5041617
Somanath Bhat, Jan Herrmann, Paul Armishaw, Philippe Corbisier, Kerry R. Emslie, Single molecule detection in nanofluidic digital array enables accurate measurement of DNA copy number Analytical and Bioanalytical Chemistry. ,vol. 394, pp. 457- 467 ,(2009) , 10.1007/S00216-009-2729-5