Polyethylenimine‐cationized β‐catenin protein transduction activates the Wnt canonical signaling pathway more effectively than cationic lipid‐based transduction

摘要: The Wnt canonical signaling pathway is essential for the early development of eukaryotic organisms and plays a key role in cell proliferation, differentiation, oncogenesis. Moreover, contributes to self-renewal mouse hematopoietic stem cells (HSCs). Here, we demonstrate artificial activation by beta-catenin protein transduction. Constitutively active was introduced into human embryonic kidney HEK-293 using polyethylenimine (PEI) cationization method, or with BioPORTER transduction reagent. We have previously shown that modification PEI effectively causes proteins be internalized living mammalian cells. PEI-cationized, constitutively added cells, induction several Wnt/beta-catenin target genes detected real-time PCR. However, introduce did not activate pathway. Introduction eGFPNuc (enhanced green fluorescent variant containing nuclear localization signal) reagent caused significant death, as determined propidium iodide staining. In contrast, PEI-modified impair survival These results indicate could successfully activated PEI-cationized beta-catenin, PEI-cationization method an effective safe technology