Protein delivery of caspase-3 induces cell death in malignant C6 glioma, primary astrocytes and immortalized and primary brain capillary endothelial cells
作者： Birgit Zassler , Ingolf E. Blasig , Christian Humpel
DOI: 10.1007/S11060-004-1364-4
关键词:
摘要: Most brain tumors consist of transformed glia cells and are highly vascularized by capillary endothelial cells. The aim the present study therefore was to deliver pro-apoptotic caspase-3 into malignant C6 glioma immortalized rBCEC4 induce cell death. Both lines were transfected with a reporter protein (β-galactosidase) using lipid-mediated gene transfer (FuGENE6TM) or novel delivery reagent BioPORTERTM. β-Galactosidase successfully delivered both cells, expression peaked around day 2 transient. Delivery induced TUNEL-positive death types. As control, also non-neoplastic primary astrocytes In conclusion BioPORTERTM-protein molecules may provide potent tool cause in tumors, however, this method is limited due its toxicity non-malignant
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