Characterization of HCV-like particles produced in a human hepatoma cell line by a recombinant baculovirus.
作者： Eiko Matsuo , Hideki Tani , Chang kweng Lim , Yasumasa Komoda , Toru Okamoto
DOI: 10.1016/J.BBRC.2005.12.001
关键词: Cellular Tropism 、 Buoyant density 、 Antibody 、 Hepatoma cell line 、 Cell culture 、 Virus-like particle 、 Biology 、 Recombinant baculovirus 、 Hepatitis C virus 、 Molecular biology
摘要: Although processing of the hepatitis C virus (HCV) polyprotein and characterization each its viral proteins have been described in detail, analysis structure assembly HCV particles has hampered by lack a robust cell culture system to support efficient replication HCV. In this study, we generated HCV-like (HCV-LP) using recombinant baculovirus encoding structural part non-structural human hepatoma line. The HCV-LP exhibited buoyant density 1.17 g/ml CsCl equilibrium gradient 40 50 nm diameter. Binding cells was partially inhibited treatment with anti-hCD81 antibody, contrast hCD81-independent binding produced insect cells. These results indicate that different types exhibit cellular tropism for target
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