Intrathecally produced IgG and IgM antibodies to recombinant VlsE, VlsE peptide, recombinant OspC and whole cell extracts in the diagnosis of Lyme neuroborreliosis

摘要: Detection of intrathecally produced specific antibodies (AI) is essential in the diagnosis Lyme neuroborreliosis (LNB); however, performance various newer AI detection methods has not been systematically assessed. Here we assessed and compared advanced test systems for detecting borrelia IgG-AI IgM-AI. Serum cerebrospinal fluid (CSF) samples from well-defined LNB tick-borne encephalitis (TBE) patients, 25 each, were tested with three antibody systems, one based on chemiluminescence (CLA) two enzyme-linked immunosorbent assays (ELISA), employing different antigens IgG IgM antibodies. In patients LNB, was detected 20 by CLA, 19 ELISA1, 22 ELISA2, IgM-AI 16 six 11 ELISA2. TBE positive case CLA 7 cases whereas none ELISA. within first week disease. Duration disease correlated while results heterogeneous each assay. Moreover, levels IgG-AI, but IgM-AI, protein concentration CSF. relevant immunoglobulin isotype intrathecal synthesis The highest sensitivity specificity achieved assay using VlsE IR6 peptide. yielded heterogenous did support laboratory LNB.