Biomolecular characterization of exosomes released from cancer stem cells: Possible implications for biomarker and treatment of cancer

摘要: Cancer recognized as one of the leading irrepressible health issues is contributing to increasing mortality-rate day-by-day. The tumor microenvironment an important field cancer understand detection, treatment and prevention cancer. Recently, stem cell (CSC) research has shown promising results aiming towards diagnostics treatment. Here, we found that prostate breast cells secreted vesicles endosomal origin, called exosomes showed strong connection between autophagy released from CSCs. Exosomes may serve communicate with neoplastic (autocrine paracrine manner) normal (paracrine endocrine thereby suppress immune systems regulate growth, metastasis. They can also be used biomarkers for various cancers. We detected tetraspanin proteins (CD9, CD63, CD81), Alix susceptibility gene-101 (TSG101) exosomal markers rotenone treated have induction genes, Atg7 conversion marker (LC3-I LC3-II), CD81) in CSCs by western blotting. mRNA expression CD9, CD81 TSG101 analyzed qRT-PCR induced Electron microscopy mitochondrial damage confirmed release constituents useful mechanism formation, function, a biomarker provide novel therapeutic strategies