Kinetin Is the Most Effective Cytokinin on Shoot Multiplication from Cucumber
作者： Saeid M. Abu-Romman , Khaldoun A. Al-Hadid , Abdullah R. Arabiyyat
关键词: Explant culture 、 Cytokinin 、 Botany 、 Shoot 、 Micropropagation 、 Cucumis 、 Biology 、 Kinetin 、 Murashige and Skoog medium 、 Zeatin
摘要: In vitro shoot multiplication of cucumber ( Cucumis sativus ) was examined from the nodal explants 10-day-old aseptic plantlets using Murashige and Skoog (MS) media supplemented with different concentration (0, 0.5, 1, 2, 3 mg L -1 cytokinins (6-Benzylaminopurine-BAP, Kinetin-Kn, Thidiazuron-TDZ, Zeatin). Nodal showed induction in response to all tried. MS medium containing Kn most effective for inducing shoots cucumber. The maximum rate regeneration (83%), highest number obtained (7.93 shoots/explant) longest (3.61 cm) were on fortified 1 Kn. lowest culture responses recorded either BAP or Zeatin. conclusion, is strongly recommended than Zeatin, TDZ obtain percentage regeneration, shoot/explant, length
cabdirect.org
sci-hub.se 参考文章(42)
F. Eudes, S. Acharya, A. Laroche, L.B. Selinger, K.-J. Cheng, A novel method to induce direct somatic embryogenesis, secondary embryogenesis and regeneration of fertile green cereal plants Plant Cell Tissue and Organ Culture. ,vol. 73, pp. 147- 157 ,(2003) , 10.1023/A:1022800512708
Carol A. Auer, Cytokinin conjugation : recent advances and patterns in plant evolution Plant Growth Regulation. ,vol. 23, pp. 17- 32 ,(1997) , 10.1023/A:1005998307340
G. Kalloo, B. O. Bergh, Genetic improvement of vegetable crops Pergamon Press. ,(1993)
G. Ananthakrishnan, X. Xia, C. Elman, S. Singer, H. S. Paris, A. Gal-On, V. Gaba, Shoot production in squash ( Cucurbita pepo) by in vitro organogenesis Plant Cell Reports. ,vol. 21, pp. 739- 746 ,(2003) , 10.1007/S00299-003-0584-Y
K. RAJASEKARAN, M. G. MULLINS, Y. NAIR, Flower formation in vitro by hypocotyl explants of cucumber (Cucumis sativus L.) Annals of Botany. ,vol. 52, pp. 417- 420 ,(1983) , 10.1093/OXFORDJOURNALS.AOB.A086592
H. Lou, S. Kako, Somatic Embryogenesis and Plant Regeneration in Cucumber Hortscience. ,vol. 29, pp. 906- 909 ,(1994) , 10.21273/HORTSCI.29.8.906
John R. Deakin, G. W. Bohn, Thomas W. Whitaker, Interspecific hybridization in cucumis Economic Botany. ,vol. 25, pp. 195- 211 ,(1971) , 10.1007/BF02860080
N. Selvaraj, A. Vasudevan, M. Manickavasagam, A. Ganapathi, In vitro organogenesis and plant formation in cucumber Biologia Plantarum. ,vol. 50, pp. 123- 126 ,(2006) , 10.1007/S10535-005-0085-7
Toshio Murashige, Folke Skoog, A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures Physiologia Plantarum. ,vol. 15, pp. 473- 497 ,(1962) , 10.1111/J.1399-3054.1962.TB08052.X
P. Sangeetha, P. Venkatachalam, Induction of direct shoot organogenesis and in vitro flowering from shoot tip explants of cucumber ( Cucumis sativus L. cv. ‘Green long’) In Vitro Cellular & Developmental Biology – Plant. ,vol. 50, pp. 242- 248 ,(2014) , 10.1007/S11627-013-9571-3