MiR-30a upregulates BCL2A1, IER3 and cyclin D2 expression by targeting FOXL2
作者： TAIREN WANG , FEI LI , SHENGJIAN TANG
DOI: 10.3892/OL.2014.2723
关键词: Carcinogenesis 、 Cell cycle 、 Gene knockdown 、 Cyclin D2 、 microRNA 、 Oncogene 、 Cyclin A 、 Immunology 、 Cancer research 、 Biology 、 Ovarian Granulosa Cell
摘要: FOXL2 is a transcription factor that essential for ovarian development. Somatic mutations of are associated with granulosa cell tumorigenesis. In the present study, expression was suppressed by microRNAs using Ago2 knockdown method in COV434 cells. Online bioinformatics tools were utilized to predict may be repressed miR-30 family members, and dual luciferase assay western blotting performed demonstrate target gene miR-30a, which relatively abundant Furthermore, miR-30a overexpression upregulates BCL2A1, IER3 cyclin D2 inhibiting FOXL2. known function as tumor suppressor breast cancer, small lung cancer colorectal carcinoma; however, study revealed an opposing oncogene.
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