Seeing the Wood through the Trees: A Review of Techniques for Distinguishing Green Fluorescent Protein from Endogenous Autofluorescence

摘要: The green fluorescent protein (GFP) from the jellyfish Aequorea victoria has recently leapt to prominence within numerous biological fields. Interest in use of GFP grown enormously over past 5 years. number papers concerning held Web Science database, which abstracts scientific literature (http://wos.mimas.ac.uk), rose 13 period 1981–1994, 3400 1995 October 2000. This is due ability clone and heterologously express genes a diverse range cells organisms, bacteria yeast, plants mammals, coupled with favorable properties such as high stability, minimal toxicity, noninvasive detection, generate highly visible fluorophore vivo absence external cofactors. Thus, become truly versatile marker for visualizing physiological processes, monitoring subcel-