Immunocytochemical differential diagnosis of adrenocortical neoplasms using the monoclonal antibody D11

摘要: The monoclonal antibody D11 is a valuable aid in the accurate typing of adrenal tumours as, formalin-fixed, paraffin-embedding material, strong nuclear positivity was observed only adrenocortical cells 190 neoplasms (including 100 tumours). This pattern demonstrated for all zona glomerulosa 27 normal adrenals and neoplastic 15 adenomas derived from that zone, as judged clinically evident hyperaldosteronism. Normal fasciculata reticularis also showed diffuse immunostaining same plus cytoplasmic reactivity benign malignant these zones, documented by hypercortisolism. Cytoplasmic and/or staining made topohistogenetic possible non-functioning cortical tumours. gave negative results 50 specimens containing normal, hyperplastic adrenomedullary cells. In addition, absence recorded 4 metastases extra-adrenal carcinomas, 5 paragangliomas, 25 primary renal carcinomas 59 60 thyroid carcinomas. immunocytochemistry allows neoplasms, irrespective histology function. With this method, can be separated metastatic to gland, including secondary similar phenotype (such carcinomas). By exclusion, negativity provides evidence medullary origin even clinical, structural, histochemical conventional immunohistochemical indicators phaeochromocytoma.