In Vitro Reconstitution of Eukaryotic Ion Channels Using Droplet Interface Bilayers

摘要: The ability to routinely study eukaryotic ion channels in a synthetic lipid environment would have major impact on our understanding of how different lipids influence channel function. Here, we describe straightforward, detergent-free method for the vitro reconstitution and ionotropic receptors into droplet interface bilayers measure their electrical activity at both macroscopic single-channel level. We explore general applicability this by from wide range sources including recombinant cell lines native tissues, as well preparations that are difficult conventional methods erythrocytes mitochondria.