Trapping cDNAs encoding secreted proteins from the salivary glands of the malaria vector Anopheles gambiae.

摘要: The signal sequence trap method was used to isolate cDNAs corresponding proteins containing secretory leader peptides and whose genes are expressed specifically in the salivary glands of malaria vector Anopheles gambiae. Fifteen unique cDNA fragments, ranging size from 150 550 bp, were isolated sequenced a first round immunoscreening COS-7 cells. All but one contained putative sequences at their 5' ends, suggesting that they likely encode secreted or transmembrane proteins. Expression analysis by reverse transcription-PCR showed least six fragments glands. Fragments showing high degree similarity D7 apyrase, two gland-specific previously found Aedes aegypti, identified. Of interest, three different D7-related represent new gene family An. Moreover, do not show known databases identified, full length cloned sequenced. RNA situ hybridization whole female patterns expression overlap only part those observed culicine mosquito A. aegypti.