Cloning and expression of apical membrane water channel of rat kidney collecting tubule
作者： Kiyohide Fushimi , Shinichi Uchida , Yukichi Harat , Yukio Hirata , Fumiaki Marumo
DOI: 10.1038/361549A0
关键词: Aquaporin 2 、 Biology 、 Cell biology 、 Xenopus 、 Tubule 、 Apical membrane 、 Endocrinology 、 Vasopressin 、 Kidney 、 Nephron 、 Exocytosis 、 Internal medicine
摘要: Concentrating urine is mandatory for most mammals to prevent water loss from the body. Concentrated produced in response vasopressin by transepithelial recovery of lumen kidney collecting tubule through highly water-permeable membranes. In this nephron segment, regulates permeability endo- and exocytosis channels or apical membrane. CHIP28 a channel red blood cells proximal tubule, but it not expressed tubule. Here we report cloning complementary DNA WCH-CD, membrane WCH-CD 42% identical amino-acid sequence CHIP28. transcripts are detected only kidney. Immunohistochemically, localized region cells. Expression Xenopus oocytes markedly increases osmotic permeability. The functional expression limited localization suggest that vasopressin-regulated channel.
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