Recent improvements in the production of antibody-secreting hybridoma cells

作者: Garry A Neil , Howard B Urnovitz

DOI: 10.1016/0167-7799(88)90075-3

关键词: AntigenAntibodyCell fusionComputational biologyMonoclonal antibodyAnticorps monoclonalNanotechnologyBiotechnologyBioengineering

摘要: Abstract The field of cell fusion technology has exploded over the past ten years, revolutionizing biology and medicine. Today, monoclonal antibodies specific for almost any antigen can be generated, setting stage many different applications. Continued research that focuses on refining developing innovative techniques will enable to reach its full potential.

参考文章(24)
M.R. Suresh, A.C. Cuello, C. Milstein, Bispecific monoclonal antibodies from hybrid hybridomas. Methods in Enzymology. ,vol. 121, pp. 210- 228 ,(1986) , 10.1016/0076-6879(86)21019-8
R. G. Lynch, J. Fleischman, H. B. Urnovitz, Yung Chang, M. Scott, IgA:IgM and IgA:IgA hybrid hybridomas secrete heteropolymeric immunoglobulins that are polyvalent and bispecific. Journal of Immunology. ,vol. 140, pp. 558- 563 ,(1988)
J A Berzofsky, H Kawamura, Enhancement of antigenic potency in vitro and immunogenicity in vivo by coupling the antigen to anti-immunoglobulin. Journal of Immunology. ,vol. 136, pp. 58- 65 ,(1986)
Don M. Wojchowski, Arthur J. Sytkowski, Hybridoma production by simplified avidin-mediated electrofusion Journal of Immunological Methods. ,vol. 90, pp. 173- 177 ,(1986) , 10.1016/0022-1759(86)90073-6
MARC SHULMAN, C. D. WILDE, GEORGES KÖHLER, A better cell line for making hybridomas secreting specific antibodies Nature. ,vol. 276, pp. 269- 270 ,(1978) , 10.1038/276269A0
Robert I. Mishell, Richard W. Dutton, IMMUNIZATION OF DISSOCIATED SPLEEN CELL CULTURES FROM NORMAL MICE Journal of Experimental Medicine. ,vol. 126, pp. 423- 442 ,(1967) , 10.1084/JEM.126.3.423
Alexis Carrel, Ragnvald Ingebrigtsen, THE PRODUCTION OF ANTIBODIES BY TISSUES LIVING OUTSIDE OF THE ORGANISM. Journal of Experimental Medicine. ,vol. 15, pp. 287- 291 ,(1912) , 10.1084/JEM.15.3.287
Ulrich Hämmerling, Tadao Aoki, Etienne de Harven, Edward A. Boyse, Lloyd J. Old, USE OF HYBRID ANTIBODY WITH ANTI-γG AND ANTI-FERRITIN SPECIFICITIES IN LOCATING CELL SURFACE ANTIGENS BY ELECTRON MICROSCOPY Journal of Experimental Medicine. ,vol. 128, pp. 1461- 1473 ,(1968) , 10.1084/JEM.128.6.1461
Mathew M. S. Lo, Tian Yow Tsong, Mary K. Conrad, Stephen M. Strittmatter, Lynda D. Hester, Solomon H. Snyder, Monoclonal antibody production by receptor-mediated electrically induced cell fusion Nature. ,vol. 310, pp. 792- 794 ,(1984) , 10.1038/310792A0