Enhanced differentiation and mineralization of human fetal osteoblasts on PDLLA containing Bioglass® composite films in the absence of osteogenic supplements

摘要: This study investigates the cellular response of fetal osteoblasts to bioactive resorbable composite films consisting a poly-D,L-lactide (PDLLA) matrix and glass 45S5 Bioglass® (BG) particles at three different concentrations (0% (PDLLA), 5% (P/BG5), 40% (P/BG40)). Using scanning electron microscopy (SEM) we observed that cells were less spread elongated on PDLLA P/BG5, whereas P/BG40 but with multiple protrusions spreading over BG particles. Vinculin immunostaining revealed similar distribution focal adhesion contacts all independent substratum, indicating materials permitted cell adhesion. However, when differentiation maturation was examined, incorporation within found significantly (p < 0.05) enhance alkaline phosphatase enzymatic activity osteocalcin protein synthesis compared tissue culture polystyrene controls alone. Alizarin red staining indicated extracellular mineralization both P/BG5 P/BG40, more bone nodules formed than PDLLA. Real time RT-PCR expression sialoprotein also affected by containing controls, Collagen Type I not influenced. By performing these investigations in absence osteogenic factors it appears stimulates osteoblast matrix, demonstrating osteoinductive capacity composite. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res,