Characterization and induction of cementoblast cell proliferation by bioactive glass nanoparticles.

摘要: Cementum is a mineralized tissue that lines the surface of tooth root enabling attachment periodontal ligament to and surrounding alveolar bone. Studies examining mechanisms involved in formation cementum have been hindered by an inability isolate culture cells required for production (cementoblasts). This study isolated characterized cementoblast derived from rat molar ligament. It was observed expressed F-Spondin, marker, while F-Spondin expression not other tissues such as gingival fibroblasts osteoblasts. As expected, also osteocalcin (OC), bone sialoprotein (BSP), alkaline phosphatase (ALP), type I collagen, demonstrating presence genes cells. These showed high ALP activity calcified nodule vitro. Since cementogenesis could be critical event regeneration tissues, this investigated whether bioactive glass particles affect proliferation cementoblasts since they are known enhance osteoblast proliferation. found ionic products nanoparticles increased viability, mitochondrial activity, induced cell Together, these results show characterization Additionally, it shown proliferate, indicating potential material use cement through engineering.