VEGF-Mediated Elevated Intracellular Calcium and Angiogenesis in Human Microvascular Endothelial Cells In Vitro Are Inhibited by Dominant Negative TRPC6
作者： M.A. Hamdollah Zadeh , Catherine A. Glass , Anette Magnussen , Jules C. Hancox , David O. Bates
DOI: 10.1080/10739680802220323
关键词: TRPC 、 Calcium 、 Vascular endothelial growth factor 、 Cell biology 、 Angiogenesis 、 Calcium in biology 、 Vascular permeability 、 Biology 、 Endothelial stem cell 、 TRPC3
摘要: Objective: Vascular endothelial growth factor (VEGF)-induced vascular permeability has been shown to be dependent on calcium influx, possibly through a transient receptor potential cation channel (TRPC)-mediated with properties of the TRPC3/6/7 subfamily. To investigate further involvement this subfamily, we determined effects dominant negative TRPC6 overexpression VEGF-mediated changes human microvascular cell (HMVEC) calcium, proliferation, migration, and sprouting. Methods: Cytoplasmic concentration was estimated by fura-2 fluorescence spectrophotometry, migration Boyden chamber assay, sprouting immunofluorescence imaging stimulated cells, proliferation flow cytometry. Results: Overexpression construct in HMVECs inhibited increases cytosolic sprouting, proliferation. In contrast, wild-type increased prolifera...
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