The Rep proteins encoded by alphasatellites restore expression of a transcriptionally silenced green fluorescent protein transgene in Nicotiana benthamiana.
作者： Qamar Abbas , Imran Amin , Shahid Mansoor , Muhammad Shafiq , Michael Wassenegger
DOI: 10.1007/S13337-017-0413-5
关键词: Cell biology 、 Nicotiana benthamiana 、 Biology 、 Green fluorescent protein 、 Gene 、 Alphasatellite 、 Transgene 、 RNA interference 、 Potato virus X 、 Northern blot
摘要: Alphasatellites are non-essential satellite-like components associated with geminiviruses. The precise selective advantage to a geminivirus infection of an alphasatellite remains unclear. ability the cotton leaf curl Multan (CLCuMuA)-encoded replication-associated protein (Rep) suppress TGS was investigated by using Nicotiana benthamiana line 16-TGS (16-TGS) harbouring transcriptionally silenced green fluorescent (GFP) transgene. Inoculation plants recombinant Potato virus X (PVX) vector carrying CLCuMuA Rep resulted in restoration GFP expression. Northern blot analysis confirmed that observed fluorescence mRNA accumulation. PVX vectors further six proteins, encoded genetically distinct alphasatellites, were similarly shown result restored These results indicate alphasatellite-encoded can restore expression transgene N. benthamiana, indicating alphasatellites involved overcoming host defence.
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sci-hub.se 参考文章(27)
J. Kumar, J. Kumar, S. P. Singh, R. Tuli, Association of satellites with a mastrevirus in natural infection: complexity of Wheat dwarf India virus disease Journal of Virology. ,vol. 88, pp. 7093- 7104 ,(2014) , 10.1128/JVI.02911-13
Imran Amin, Basavaprabhu L Patil, Rob W Briddon, Shahid Mansoor, Claude M Fauquet, Comparison of phenotypes produced in response to transient expression of genes encoded by four distinct begomoviruses in Nicotiana benthamiana and their correlation with the levels of developmental miRNAs Virology Journal. ,vol. 8, pp. 238- 238 ,(2011) , 10.1186/1743-422X-8-238
Javaria Qazi, Imran Amin, Shahid Mansoor, Muhammad Javed Iqbal, Rob W. Briddon, Contribution of the satellite encoded gene βC1 to cotton leaf curl disease symptoms Virus Research. ,vol. 128, pp. 135- 139 ,(2007) , 10.1016/J.VIRUSRES.2007.04.002
Muhammad Shah Nawaz-ul-Rehman, Nazia Nahid, Shahid Mansoor, Rob W. Briddon, Claude M. Fauquet, Post-transcriptional gene silencing suppressor activity of two non-pathogenic alphasatellites associated with a begomovirus Virology. ,vol. 405, pp. 300- 308 ,(2010) , 10.1016/J.VIROL.2010.06.024
S. Sánchez-Campos, A. Martínez-Ayala, B. Márquez-Martín, L. Aragón-Caballero, J. Navas-Castillo, E. Moriones, Fulfilling Koch's postulates confirms the monopartite nature of tomato leaf deformation virus: A begomovirus native to the New World Virus Research. ,vol. 173, pp. 286- 293 ,(2013) , 10.1016/J.VIRUSRES.2013.02.002
Rob W Briddon, Simon E Bull, Imran Amin, Shahid Mansoor, Ian D Bedford, Narayan Rishi, Surender S Siwatch, Yusuf Zafar, Aly M Abdel-Salam, Peter G Markham, Diversity of DNA 1: a satellite-like molecule associated with monopartite begomovirus–DNA β complexes Virology. ,vol. 324, pp. 462- 474 ,(2004) , 10.1016/J.VIROL.2004.03.041
Muhammad Saeed, Gabi Krczal, Michael Wassenegger, Three gene products of a begomovirus–betasatellite complex restore expression of a transcriptionally silenced green fluorescent protein transgene in Nicotiana benthamiana Virus Genes. ,vol. 50, pp. 340- 344 ,(2015) , 10.1007/S11262-014-1155-8
S Chapman, T Kavanagh, D Baulcombe, Potato virus X as a vector for gene expression in plants Plant Journal. ,vol. 2, pp. 549- 557 ,(1992) , 10.1046/J.1365-313X.1992.T01-24-00999.X
Nathan Pumplin, Olivier Voinnet, RNA silencing suppression by plant pathogens: defence, counter-defence and counter-counter-defence Nature Reviews Microbiology. ,vol. 11, pp. 745- 760 ,(2013) , 10.1038/NRMICRO3120
Athanasios Dalakouras, Michael Wassenegger, Revisiting RNA-directed DNA methylation RNA Biology. ,vol. 10, pp. 453- 455 ,(2013) , 10.4161/RNA.23542