Induction of heat shock protein 70 and nucleolin and their intracellular distribution during early stage of liver regeneration.

摘要: Our previous work demonstrated a simultaneous induction of Hsp70 and nucleolin mRNA during the prereplicative stage hepatocytes after partial hepatectomy [Ohmori, H. et al. (1990) Exp. Cell Res. 189, 227-232]. In present study, changes intracellular localization these proteins were examined. Nucleolin, which mainly localized in nucleus, increased with time accumulated nucleolus around 12-18 h hepatectomy. protein also slightly 6-12 operation. accordance this increase, immunohistochemical staining revealed that almost all nuclei became Hsp70-positive, although was seen to be dispersed throughout nucleoplasm at times Next, we isolated cDNA clone ribosomal (S-17) examined its behavior. Induction S-17 observed essentially similar regenerating rat liver, inductions histone H2A H4 occurred later time, is, parallel DNA synthesis. Furthermore, by serum-stimulation serum-depletion HeLa IAR-20 (rat) culture cells. These results suggest Hsp70, addition nucleolin, not fortuitous but may involved early events liver regeneration.