Characterization of Human Brain S100 Protein Fraction: Amino Acid Sequence of S100?
作者： Roy Jensen , Daniel R. Marshak , Clint Anderson , Thomas J. Lukas , D. Martin Watterson
DOI: 10.1111/J.1471-4159.1985.TB04048.X
关键词: Amino acid 、 Peptide sequence 、 Protein purification 、 Trypsin 、 Human brain 、 S100 protein 、 Biochemistry 、 Cyanogen bromide 、 Cleavage (embryo) 、 Biology
摘要: Abstract Two major components of human brain S100 fraction were purified by HPLC and an amino acid sequence was elucidated for the beta component. Human proteins showed absorption spectra compositions similar to alpha from bovine brain. However, relative amounts 4% 96% weight, while protein distribution 47% 53% weight. An established analysis overlapping fragments generated cyanogen bromide trypsin cleavage. Three differences between found at residues 7, 62, 80. These chemically conservative compatible with minimum single base changes in codon structures. results document that is a conserved among mammals provide necessary foundation current clinical studies.
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