Effects of calcitriol (1, 25-dihydroxy-vitamin D3) on the inflammatory response induced by H9N2 influenza virus infection in human lung A549 epithelial cells and in mice

摘要: H9N2 influenza viruses circulate globally and are considered to have pandemic potential. The hyper-inflammatory response elicited by these is thought contribute disease severity. Calcitriol plays an important role in modulating the immune viral infections. However, its unknown whether calcitriol can attenuate inflammatory virus infection. Human lung A549 epithelial cells were treated with (100 nM) then infected virus, or (30 nM). Culture supernatants collected every 24 h post infection growth kinetics evaluated. (5 mg/kg) was administered daily intraperitoneal injection BABL/c mice for 15 days following Mice monitored clinical signs of disease, pathology responses. treatment prior significantly decreased expression M gene, IL-6, IFN-β cells, but did not affect replication. In vivo, we found that downregulated pulmonary inflammation 2 days post-infection, increased 4 6 days post-infection. contrast, antiviral cytokine higher calcitriol-treated than untreated at lower on days 8 elevated levels pro-inflammatory cytokines consistent period maximum body weight loss damage mice. These results suggest might a negative impact innate mice, especially later stage This study will provide some novel insights into use modulate humans.