Colloidal gold labelling of l,4‐β‐D‐glucan cellobiohydrolase adsorbed on cellulose substrates
作者： H. Chanzy , B. Henrissat , R. Vuong
DOI: 10.1016/0014-5793(84)81124-2
关键词: Adsorption 、 Crystallography 、 Cellulose 、 Trichoderma reesei 、 Enzyme 、 Chemistry 、 Labelling 、 Colloidal gold 、 β d glucan 、 Crystal
摘要: The 1,4-β-D-glucan cellobiohydrolase I (CBHI) from Trichoderma reesei was labelled with 4–6 nm gold particles. This CBHI-gold complex still retains 60% of the original CBHI activity and allows good visualization enzyme adsorbed at surface cellulose mirofibrils or microcrystals. main features adsorption are: rapid irreversible binding; preference crystal edges instead for absence specificity tips where chain ends are supposedly located.
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