Using a 3D virtual muscle model to link gene expression changes during myogenesis to protein spatial location in muscle

摘要: Background: Myogenesis is an ordered process whereby mononucleated muscle precursor cells (myoblasts) fuse into multinucleated myotubes that eventually differentiate myofibres, involving substantial changes in gene expression and the organisation of structural components cells. To gain further insight orchestration these we have overlaid spatial protein a cell with their during differentiation using new 3D visualisation tool: Virtual Muscle (VMus3D). Results: Sets generic striated costamere, Z-disk filament proteins were constructed from literature protein-interaction databases. Expression profiles genes encoding obtained mouse C2C12 undergoing myogenesis vitro, as well tissue survey dataset. Visualisation data VMus3D yielded novel observations significant relationships between location temporal products genes. A specificity index was calculated based on relative to median all tissues and, expected, highest also expressed most dynamically differentiation. Interestingly, costamere some appeared be broadly across showed little change differentiation, line broader cellular role described for proteins. Conclusion: By studying patterns perspective demonstrated not are part specific structures simply up-regulated Indeed, group whose program appears minimally affected by process, code participating vital skeletal structures. alone poor metric behaviour. Instead, "connectivity model development" proposed mechanism development: closer myofibril core cells, greater specificity.