Correlation of dynamic contrast enhancement MRI parameters with microvessel density and VEGF for assessment of angiogenesis in breast cancer

摘要: Purpose To investigate the association between parameters obtained from dynamic contrast enhanced MRI (DCE-MRI) of breast cancer using different analysis approaches, as well their correlation with angiogenesis biomarkers (vascular endothelial growth factor and vessel density). Materials Methods DCE-MRI results were 105 patients (108 lesions). Three methods applied: 1) whole tumor analysis, 2) regional hot-spot 3) intratumor pixel-by-pixel analysis. Early enhancement intensities fitted pharmacokinetic studied. Paraffin blocks 71 surgically resected specimens analyzed by immunohistochemical staining to measure microvessel counts (with CD31) vascular (VEGF) expression levels. Results MRI three showed significant correlations (P < 0.0001), but a substantial dispersion linear regression line was noted (r = 0.72–0.97). The entire region interest (ROI) vs. pixel population analyses had significantly higher compared ROI analyses. Cancer high VEGF CD31 densities than did low levels 0.005). No found strategies IHC based measurements angiogenesis. Conclusion A consistent strategy important in DCE-MRI study. In this series, none these yielded for quantitation vascularity that associated measurements. Therefore, could not account lack association. J. Magn. Reson. Imaging 2003;18:467–477. © 2003 Wiley-Liss, Inc.