The role of accessory cells in polyclonal T cell activation II. Induction of interleukin 2 responsiveness requires cell-cell contact

摘要: It has previously been reported (Hunig, T. et al., Eur. J. Immunol. 1983. 13:1) that highly purified peripheral T cells do not respond to concanavalin A (Con A) even in the presence of Con A-induced spleen cell supernatant as a source interleukin 2 (IL 2). In present report, hypothesis was tested whether this unresponsiveness correlates with observed inability mediate cell-cell contact between cells. found cells, pretreated neuraminidase reduce their net negative charge, were both aggregated and rendered IL 2-reactive by A. addition, leukoagglutinin (LA) be able agglutinate untreated make them 2-reactive. Neuraminidase treatment reduced concentration LA required effects. did alter overall A-binding capacity nor it induce reactivity absence lectin. Furthermore, irradiated, neuraminidase-treated served accessory (AC) induction responsiveness 2, but for production IL2, which depends on Ia+ AC. Finally, Lyt-2- responded same fashion whole populations, indicating at least some helper phenotype need interact Ia+AC responsiveness.